Effects of Bushen Chinese Medicine on Expression of Cbf?1 in Aging Osteoblast of Rat
- VernacularTitle:补肾中药对增龄大鼠成骨细胞Cbf?_1表达的影响
- Author:
Weiwei WANG
;
Yiyong WEI
;
Yinyu SHI
- Publication Type:Journal Article
- Keywords:
Bushen Chinese medicine;
core-binding factor ?1;
osteoblast;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of core-binding factor ?1 (Cbf?1) in aging osteoblast, and try to provide new mechanisms of Chinese medicine on bone formation. Methods Rat osteoblast from different age donors (6 months and 16 months) were isolated and cultured by the tissue-fragment-migrating-growth method. The biological characteristic was observed by phase-contrast microscope and identified with mineralizing nodule staining. The expression of Cbf?1 was examined by RT-PCR and immunohistochemistry. Results RT-PCR and immunohistochemistry showed that the expression of Cbf?1 was upregulated by Gubenzhuanggu and Jinkuishenqi, and downregulated by Zhibaidihuang in aging osteoblast, but the effect was different on the Cbf?1 expression in Bushenyijing. Conclusion Buyang Chinese medicine among Bushen Chinese medicine promoted bone formation.
