Effect of Hedyotis Diffusa Extract on Apoptosis of Human Hepatocarcinoma Cell Line Bel7402
- VernacularTitle:白花蛇舌草对人肝癌Bel7402细胞凋亡的影响
- Author:
Xiufang LU
;
Qingyu MENG
;
Xinmin GUO
- Publication Type:Journal Article
- Keywords:
Hedyotis diffusa extract;
Bel7402;
apoptosis;
Bcl-xl;
p53
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inducing apoptosis of Hedyotis diffusa extract (HDE) on human hepatocarcinoma cell line Bel7402 and its molecular mechanism. Methods Human hepatocarcinoma cell line Bel7402 was used in vitro cell culture, there were 3 groups:control group, HDE group and 5-Fu group, the Bel7402 cells were dealed with them respectively. The apoptosis morphologic changes of human hepatocarcinoma cells were observed by invert microscope. The apoptosis rate was detected with hematoxylin stain by light microscope. Inhibition of Bel7402 cell proliferation was measured by MTT assay. The expression of oncogene Bcl-xl and anti-oncogene p53 of Bel7402 ceils were observed with RT-PCR assay. Results Compared with the control group, the condensation of nuclear, vacuolar degeneration of mitochondria could be found through light microscope. The apoptosis rate of HDE group was remarkably increased compared with that in control group (P
