Effect of Hydroxycamptothecin on Proliferation of Human Lung Cancer Cell Line A549
- VernacularTitle:羟基喜树碱对A549人肺腺癌细胞增殖的影响
- Author:
Ying WANG
;
Chunying LIU
;
Yuanzhi GUO
- Publication Type:Journal Article
- Keywords:
Hydroxycamptothecin;
human lung cancer cell line A549;
cell calture;
MTT assay
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Hydroxycamptothecin (HCPT) on proliferation of human lung cancer cell line A549 in vitro. Method Using cell calture and MTT assay to observe the effect of HCPT on proliferation of human lung cancer cell line A549. Result Lower concentration of HCPT had no evident inhibitory effect on the human lung cancer cell line A549 after 24 h. The effect was evident when the concentration of HCPT was up to 50 ?g/mL, and the inhibitory rate was 44.17%. The inhibitory rate was 50.28% when the concentration of HCPT was up to 100 ?g/mL. The inhibitory effect of HCPT became more significant with the stimulation of the time, and the inhibitory rate of 100 ?g/mL concentration of HCPT was 70.98% after 48 h. Conclusions HCPT can inhibit the proliferation of human lung cancer cell A549 in vitro. The effect is dose and time dependent.
