AFLP Analysis on diversity of germplasm resource in cultivated and wild Cistanche deserticola
- VernacularTitle:肉苁蓉种质资源多样性的AFLP分析
- Author:
Rong XU
;
Jun CHEN
;
Shilin CHEN
;
Tongning LIU
;
Ren NA
- Publication Type:Journal Article
- Keywords:
Cistanche deserticola Y. C. Ma;
germplasm resources;
genetic diversity;
amplified fragment length polymorphism (AFLP)
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the genetic diversity of germplasm resource in cultivated and wild Cistanche deserticola. Methods Fifty-eight samples from three populations of cultivated and wild C. deserticola were analyzed by amplified fragment length polymorphism (AFLP) DNA markers, and the gene- tic diversity was evaluated by PopGen32. Results The average percentage of polymorphic loci (PPL) of cultivated C. deserticola is 79.16%. The PPL of wild population is 89.53%. Average Neis gene diversity index (He) from four populations was 0.193 8, Shannons genetic diversity index (I) was 0.300 4, and genetic differentiation index (Gst) was 0.097 9. Conclusion The diversities of cultivated and wild C. deserticola are both higher and theres no differentiation between them. It shows that genetic diversity of inner-species is higher, which is not the reason for endangerment. Therefore, wild nursery and artificial cultivating are the best measures for the conservation and sustainable utilization in C.deserticola.
