Molecular cloning,sequence analysising and repairing the mutation of mouse integrin ?_7 gene
- VernacularTitle:小鼠整合素?_7基因克隆、序列分析及突变修复
- Author:
Yongming WANG
- Publication Type:Journal Article
- Keywords:
?7 gene;
cDNA clone;
RT-PCR;
Repairing mutation
- From:
Journal of Chongqing Medical University
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone and analyze a full-length cDNA encoding mouse integrin ?7,and repair the mutation of ?7 cDNA that caused the change of amino acids. Methods:The cDNA of ?7 gene was amplified by RT-PCR using the total RNA as extracted from mouse small intestine Peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed E.coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of ?7 cDNA that caused the change of amino acids was repaired. Results:The cDNA of mouse ?7 has an complete open reading frame with a length of 2418 bp,which encodes a product of 806 amino acid,and has 10 base pairs mutation of ?7 gene and 5 base pairs mutation that caused the change of amino acids was repaired. Conclusion:The cDNA of mouse ?7 was cloned successfully,which posed a basis for further researching on its biological function.
