Study on AdCD-UPRT suicide gene mediated on lung cancer A_(549) in vitro
- VernacularTitle:融合自杀基因AdCD-UPRT对人肺癌细胞A_(549)的体外作用
- Author:
Dan XU
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Suicide gene;
Lung neoplasms;
Gene therapy
- From:
Journal of Chongqing Medical University
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. Methods:The recombinant AdCD-UPRT adenovirus was packaged and generated,and the title of virus was measured. The efficiency of transfecting target cells was detected,the toxicity of virus was measured,and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining,transmission electron microscope and flow cytometry. Results:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. Conclusion:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.
