Planting and biological character of corneal and oral epithelial cells cultivated on amniotic membrane
- VernacularTitle:兔角膜缘和口腔粘膜上皮细胞羊膜种植及生物学特性研究
- Author:
Mei XU
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Epithelial cells;
Amniotic membrane
- From:
Journal of Chongqing Medical University
2007;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the cultivation and boilogical char-acter of corneal epithelial cells(ECs) and oral ECs planted on amniot-ic membrane (AM) as a substrate. Methods:Rabbit limbus ECs were planted on denuded AM and intact AM respectively. Oral Ecs were p-lanted on denuded AM.The cultivated epithelial sheet was examined by HE staining pathological section and electronmicroscopy(EM),also immunohistochemically labeled with AE5 monoconal antibody to identify the expression of cytokeratin 3(CK3). Results:Rabbit corneal ECsgrew slowly and were difficult to stick and confluence on intact AM,while both rabbit corneal and oral ECs were easy to grow and proli-ferate on denuded AM wheather using tissue pieces or single cells.T-he resulted tissure was composed of the basement membrane of AM and multiple layers of ECs showing the presence of CK3. Conclusions:The stratification of corneal and oral epithelium layers attaching to the basement membrane of AM are similar to engineering tissue mak-ing a fundament to ocular surface reconstruction.
