Effects of Liuwei Dihuang Pills on the Proliferation and Differentiation of Rat Preadipocytes
- VernacularTitle:六味地黄丸对大鼠前脂肪细胞增殖与分化的影响
- Author:
Zizeng XIAO
;
Bing DAI
;
Lei LIU
- Publication Type:Journal Article
- Keywords:
Liuwei Dihuang pills;
rat;
preadipocytes;
cell proliferation;
cell differentiation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Liuwei Dihuang pills on proliferation and differentiation of rat preadipocytes. Methods Rat preadipocytes were cultured, the influence of Liuwei Dihuang drug-containing serum on proliferation of rat preadipocytes was detected by MTT method, the influence of Liuwei Dihuang drug-containing serum on expression of GPDH was determined by enzyme tissue chemical method, the effect on lipid accumulation was determined by Oil Red O staining. Results Liuwei Dihuang drug-containing serum stimulated rat preadipocyte proliferation, inhibited GPDH up-regulation during differentiation, inhibited lipid accumulation in cell differentiation with the concentration below 10%. Conclusion Liuwei Dihuang pills stimulated rat preadipocyte proliferation, inhibited rat preadipocyte differentiation.
