Determination of Emodin in Radix et Caulis Ventilaginis Leiocarpae by HPLC
- VernacularTitle:HPLC法测定红穿破石中大黄素的含量
- Author:
Weian HUANG
;
Yi MENG
;
Weifeng FAN
- Publication Type:Journal Article
- Keywords:
Radix et Caulis Ventilaginis leiocarpae;
emodin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determining emodin in Radix et Caulis Ventilaginis leiocarpae. Methods Stationary phase was Agilent Eclipse XDB-C18 (4.6 mm?150 mm, 5 ?m), mobile phase was methanol-0.1%phosphoric (75∶25), detection wavelength was 285 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results There was good linearity in the range of 0.006 3～0.063 ?g for emodin (r=0.999 9). The average recovery of emodin was 102.0% with RSD=1.58%. Conclusion This method is rapid and accurate. It can be applied to control the quality of Radix et Caulis Ventilaginis leiocarpae.
