Content Determination of Hyodeoxycholic Acid in Qingkailing Injection by HPLC
- VernacularTitle:HPLC法测定清开灵注射液中猪去氧胆酸的含量
- Author:
Li ZHANG
;
Jianguo MIAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Qingkailing Injection;
hyodeoxycholic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for content determination of hyodeoxycholic acid in Qingkailing Injection. Methods An UltimateXB-C18 column (5 ?m, 250 mm?4.6 mm) was used with a mobile phase of acetonitril-water-phosphoric acid (35∶65∶0.1). The flow rate was 1 mL/min. The temperature of the column was 40 ℃. The detection wavelength was 192 nm. Results There was a good linear relationship between the concentration of hyodeoxycholic acid and absorption area value in range of 0.201 35～1.006 75 mg/mL, r=0.999 6. The average recovery was 98.25% with RSD=1.26%. Conclusion This method was accurate, credible and repeatable which can be used to control the quality of Qingkailing Injection.
