Determination of Psoralen and Isopsoralen in Different Effective Extracts of Fructus Psoraleae by RP-HPLC
- VernacularTitle:补骨脂药材不同有效部位中补骨脂素和异补骨脂素的含量测定
- Author:
Xuejun HU
;
Songfeng ZHEN
;
Chaozhan LIN
;
Jinyan YANG
;
Chenchen ZHU
- Publication Type:Journal Article
- Keywords:
Fructus Psoraleae;
Psoralen;
Isopsoralen;
Effective extracts;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the assay of psoralen and isopsoralen in different effective extracts of Fructus Psoraleae. Methods HPLC was carried out on the column of Kromasil RP-C18. The mobile phase was methanol -water(65 ∶35). The flow rate was 1.0 mL/min and the UV detection wavelength was 245 nm. Results Good linearity of psoralen was showed within the range of 10.5 ng～525 ng(r= 0.999 3)and isopsoralen within the range of 9 ng～450 ng (r= 0. 999 9). The content of psoralen and isopsoralen differed in different extractions of Fructus Psoraleae. Among them,the extract C (extracted by ethyl acetate ) contained the highest contents of psoralen and isopsoralen,while the contents of psoralen and isopsoralen were very low in the extract D (extracted by n-butyl alcohol) and E (supernatant of water extract). Conclusion The method is simple,accurate and reproducible. The anti-asthma effect and the dose-effect relationship of the different effective extracts of Fructus Psoralea need further pharmacodynamics study.
