Determination of Astragaloside Ⅳ in Xiyangshen Jinqiangui Mixture by HPLC-ELSD Method
- VernacularTitle:HPLC-ELSD法测定西洋参金钱龟合剂中黄芪甲苷的含量
- Author:
Yi LUO
- Publication Type:Journal Article
- Keywords:
Xiyangshen Jinqiangui Mixture;
Astragaloside Ⅳ;
HPLC-ELSD
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a special determination method of astragaloside Ⅳin Xiyangshen Jinqiangui Mixture by HLPC-ELSD. Methods HPLC-ELSD was performed on Inertsil C18 Column(5 ?m,4.6 mm?250 mm),acetonitrile-water(30 ∶70) served as mobile phase ,column temperature was 35 ℃,the flow rate was 1.0 mL?min-1,and an evaporating light-scattering detector (ELSD) was used as detector. Results For astragaloside Ⅳ,the linear range was 0.424 4～2.122 ?g and the average recovery was 101.77 %with corresponding RSD being 2.49 %(n=6). Conclusion The method is simple,sensitive ,reproducible,accurate and can be used for the quality control of Xiyangshen Jinqiangui Mixture.
