Determination of Arctiin in Pifu Zhiyang Tablets by SPE-HPLC
- VernacularTitle:SPE-HPLC测定皮肤止痒片中牛蒡子苷的含量
- Author:
Yufan ZOU
;
Xiaogen WANG
;
Limei YAO
- Publication Type:Journal Article
- Keywords:
Pifu Zhiyang Tablets;
Arctiin;
Content determination;
SPE;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of arctiin in Pifu Zhiyang Tablets by SPE-HPLC . Methods SPE-HPLC method was performed on a Kromasil C18 column(150 mm?4.6 mm ,5 ?m),the mobile phase consisted of methanol -1 %acetic acid (35 ∶65) ,the wavelength was 283nm . Results The linear range of arctiin was 1.5～4.5 ?g(r= 0. 999 8) ,the average recovery was 98.18 %with RSD of 2.08 %. Conclusion This method is accurate ,reliable and reproducible. It can be used for the determination of arctiin in Pifu Zhiyang Tablets .
