Purification of a New Antibacterial Protein from Housefly(Musca domestica)Larvae
- VernacularTitle:家蝇幼虫体内一种抗菌蛋白的分离纯化
- Author:
Hui QU
;
Youjin HAO
;
Yingjun JING
;
Desen LI
;
Rongqian DU
- Publication Type:Journal Article
- Keywords:
Housefly Protein purification Antibacterial protein
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Insects exhibit a particular resistance to infections.The activation of the innate immune response of insects is involved in the recognition of the infectious nonself and subsequent activation of cellular and humoral reactions.In humoral reactions,insect antibacterial peptides and lysozyme are very important in resistance to infections.Housefly(Musca domestica) is one of the most important kinds of insects and it has strong ability to adapt to the adverse circumstances.It is of momentous theoretical and practical significance to research the immunity system of housefly.The methods of inducement of housefly larvae were firstly studied.Then an antibacterial protein,whose molecular weight is 28kDa,was purified from housefly larvae,induced by 30% H2O2,through saltout,Sephadex G25 column,Sephadex G75 column and CMSepharose Fast Flow column.This antibacterial peptide had activities against most of Gram positive and Gram negative bacteria.
