HPLC digitized fingerprints of Radix et rhizoma seu caulis Acanthopanacis senticosi
- VernacularTitle:刺五加HPLC数字化指纹图谱研究
- Author:
Guoxiang SUN
;
Xiuming YU
;
Kaishun BI
- Publication Type:Journal Article
- Keywords:
Radix et rhizoma set caulis Acanthopanacis senticosi;
high performance liquid chromatography;
digitized fingerprint;
chromatographic fingerprint index(F);
fingerprint quantitative similarity calculated by vector shadow C%;
apparent quantitative si
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a novel method for the overall quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi on the ground of HPLC digitized fingerprint.METHODS: The chromatographic fingerprints were obtained by injecting 10 ?L of the sample solution each time on a Century SIL BDS column(20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetate acid water and 1% acetate acid acetonitrile.The flow rate was 1 mL/min,the colunm temperature was maintained at(30?0.15)℃ and the detection wavelength was set at 265 nm. RESULTS: 31 co-possessing peaks were selected as the fingerprint peaks of Radix et rhizoma seu caulis Acanthopanacis senticosi and the similarities between the chromatographic fingerprints and the herbal drugs were calculated taking chlorogenic acid peak as the reference peak.The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index(F),the relative index(Fr). CONCLUSION: This method with good precision and reproducibility can be useful for the quality control of Radix et rhizoma seu caulis Acanthopanacis senticosi.
