Identification of Bailing Capsule by RP-HPLC fingerprint
- VernacularTitle:百令胶囊的指纹图谱鉴别研究
- Author:
Ming ZHU
- Publication Type:Journal Article
- Keywords:
Bailing Capsule;
identification;
HPLC fingerprint
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a specific method for identifying Bailing Capsule(Synnematum sinense,Yin et shen sp.now).METHODS: Using fermented cordyceps powder(C_S-C-Q80),adenosine and uridine as reference standards,a HPLC fingerprint at 260 nm has been established for identification of Bailing Capsule,using Alltima C_(18)(250 mm?4.6 mm,5 ?m) column and acetonitrile-0.04 mol/L KH_2PO_4 in gradient elution as a mobile phase.The flow rate was 0.8 ml/min. RESULTS: The relative standard deviations of stability,precision and reproducibility was less than 3% respectively.The relative standard deviation of relative retention time was less than 5% compared with other HPLC apparatuses and different colunms. CONCLUSION: The method established in this paper has the exclusivity and can be used to identify the Bailing Capsule.
