Determination of hesperidin and salvianolic acid B in Xiaopikang Capsule by HPLC
- VernacularTitle:HPLC测定消癖康中橙皮苷和丹酚酸B的含量
- Author:
Junxiang WANG
;
Dongying WANG
;
Junyong LI
- Publication Type:Journal Article
- Keywords:
Xiaopikang Capsule;
hesperidin;
salvianolic acid B
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the contents of hesperidin and salvianolic acid B in Xiaopikang Capsule(Folium Citri reticulatae,Radix et Rhizoma Salviae miltiorrhizae,etc.) by HPLC. METHODS: The HPLC system consisted of Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m), metanol-acetonitrile-formicacid-water(30∶10∶1∶59) mixture as mobile phase,detection wavelength at 284 nm,flow rate of 1.0 mL/min. RESULTS: The ca-(libration) curves of two ingredients were linear within the ranges of 0.22 ?g-2.2 ?g(r=0.999 6,hesperidin),(1.62 ?g-10.2 ?g)(r=0.999 9,salvianolic acid B),respectively.The average recoveries of hesperidin was(98.9%)(RSD=1.60%),salvianolic acid B for 99.02%(RSD=0.97%). CONCLUSION: The method is rapid,sensitive and accurate.
