The effect of transcription factor SP1 decoy oligodeoxynucleotides on expression of ? Gal in SV-40-PED cells
- VernacularTitle:转录因子SP1诱骗性寡核苷酸对猪血管内皮细胞?半乳糖苷链表达的影响
- Author:
Yabing HUANG
;
Lu WANG
;
Lin XIE
;
Tao PAN
;
Hui GUO
;
Rong LI
;
Song CHEN
;
Shi CHEN
- Publication Type:Journal Article
- Keywords:
Transcription factor;
SP1 decoy oligodeoxynucleotides;
Endothelium cell;
Transplantation;
Heterologous
- From:
Journal of Medical Postgraduates
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of transcription factor SP1 decoy oligodeoxynucleotides(ODN) on expression of ? Gal in SV-40-PED cells.Methods:Immortalized porcine aortic endothelial cells of the PED line were cultured and transfected with ?1,3galactosyltransferase(?1,3GT) specific decoy ODN.Cells transfected with mismatch ODN was used as negative controls.Twenty-six hours later the cells were collected.The expression of ? Gal was determined with fluorescence microscope and Western blot.The expression of ?1,3GT mRNA was examined by RT-PCR.Results:Fluorescence microscopy observed the decreased fluorescence of ? Gal after decoy ODN transfection.Western blot showed that the average absorbance of the PED cells transfected with decoy ODNs was(48.2?0.9).It is 52.6% of the mock group(P0.05).Conclusion:?1,3GT gene reduce actually occurs following transfection of decoy ODN.Porcine endothelial cells can be the targets of decoy ODN.
