Study of biological properties of bone marrow stromal cells in vitro in wistar rats
- VernacularTitle:体外培养大鼠骨髓基质细胞生物学特性研究
- Author:
Qin YANG
- Publication Type:Journal Article
- Keywords:
Wistar rats;
Bone marrow stromal cells;
Cell culture
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the biological properties of bone marrow stromal cells(MSCs) in vitro in Wistar rats for inducing MSCs to differentiate into neurons and transplantating MSCs to treat cerebral ischemic stroke.Methods:The Wistar rats for 4 weeks old were sacrified,the bone marrow separated and the MSCs cultured,and the biological properties of the cells observed by phasecontrast microscope.Cell surface markers were evaluated by indirect immunofluorescence cycochemistry staining in primary and different generation cells.Results:95.18% and 94.36% of the third generation cells showed positivity to CD54 and CD44(MSCs surface marker).The percentage of hematopoietic lineage marker CD14 decreased obviously from 23.19% in primal culture of the cells to 2.37% in the third generation.Conclusion:The third generation cells can be used as the seed-cells for study of cells induce and differentiation and the resoure of cells transplantation.
