Establishment and identification of siRNA eukaryotic expression vectors of recombinant targeting gene glucocorticoid receptor
- VernacularTitle:糖皮质激素受体GR真核表达载体的构建与鉴定
- Author:
Xiuying LI
- Publication Type:Journal Article
- Keywords:
Glucocorticoid receptor(GR);
RNA interference;
pBS/U6-Neo vector
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Establish eukaryotic expression vector of glucocorticoid receptor GR,to explore the relation of GR and tradition Chinese medicine.Methods:Recombinant were designed and established Supported by the National Natural Science Foundation of China(3047119)by targeting gene GR and plasmid pBS/U6-Neo based on GR mRNA sequence.Two pairs of oligonucleotides were synthesized and inserted into plasmid pBS/U6-Neo to generate siRNA eukaryotic expression vectors.DH5a strains were transformed.plasmids were extracted,and the recombinant sequences were identified by PCR and sequencing.Results:The result of recombinant sequence was the same as aim sequence.The recombinant vectors were established successfully.Conclusion:siRNA recombinant can be established successfully by RNAi technique,to use in further gene stability transfer and cultivate transgenic mouse.
