Extraction of anti-asthma activitive protein components from fresh Earthworm (Eisenia foetida)
- VernacularTitle:鲜地龙平喘活性蛋白提取工艺研究
- Author:
Zehai FANG
;
Yi FENG
;
Desheng XU
- Publication Type:Journal Article
- Keywords:
fresh Earthworm(Eisenia foetida);
anti-asthma protein;
orthogonal experiment;
extraction
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To identify the optimal extraction of anti-asthma active protein from fresh Earthworm(Eisenia foetida).METHODS:Extraction time,extraction solvent and pH were selected as influencing factors.90 quinea pigs were randomly divided into 9 groups(control,montelukast,low temp,room temp,acidic buffer extract,alkaline buffer extract,aqueous extract,neutral buffer extract,powder earthworm group).Anti-asthma effciency was compared among groups.extraction time,saturation of ammonia sulfate buffer volume were selected as factors of orthogonal design.RESULTS:The optimal extraction for anti-asthma protein was at 4 ?C,adding 2 fold volume of pH 6.8 buffer phosphate,homogenating,standing and extracting for 3 hours,taking the supernataut and centifuging,salting out with 80% ammonia sulfate,suspecting with 20 mmol/L,pH6.8 buffer phosphate.So Earthworm protein was gained from the clear supernatant.CONCLUSION:The extraction technique may keep its original activity.
