Quantitative determination of notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 contents in Xinnao Guantong Guttate Pill by HPLC gradient elution method
- VernacularTitle:RP-HPLC梯度洗脱法测定心脑贯通滴丸中三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1的含量
- Author:
Canfang LIU
;
Yaoqin WEN
;
Hong WANG
;
Shizhon CHEN
- Publication Type:Journal Article
- Keywords:
HPLC gradient elution method;
Xinnao guantong Guttate Pill;
notoginsenoside R_1;
ginsenoside Rg_1;
ginsenoside Rb_1
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:A HPLC linear gradient elution method was studied for determining notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 content in Xinnao guantong Guttate Pill.METHODS:The YMC-Pack ODS-A column was used with a mobile phase of acetonitrile-water 0-15 min(23∶77),16-40 min(50∶50)gradient elution,the wavelength of detecter was set at 203 nm.RESULTS:The linear ranges of R_1,Rg_1,Rb_1 were 0.204-1.836,0.818-7.362,0.858-7.722 ?g,respectively.The average recoveries of R_1,Rg_1,Rb_1 were 97.86%,101.71%,101.44%,respectively.CONCLUSION:The method is rapid,accurate,sensitive and reliable.The results show that method can be used to control quality of products.
