Determination of geniposide and baicalin in four preparations by HPLC
- VernacularTitle:HPLC测定4种中药制剂中栀子苷和黄芩苷的含量
- Author:
Weijia LI
;
Qiang WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
geniposide;
baicalin;
Chinese medicine preparation
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To established a method for determining geniposide and baicalin in four preparations cantaining Fructus gardeniae and Radix Scutellariae by HPLC.METHODS:The separation was performed on Lichrospher C_ 18 column;using water-phosphoric acid(53∶0.2)and acetonitrile as a mobile phase,gradient elution.The detection wavelength was 240 nm.The flow rate was at 1.0 mL/min.The column temperature was at room temperature.RESULTS:The linear range of geniposide was in the range of 1.9-76 ?g/mL,r=0.999 9,the average recovery was 101.3%.The linear range of baicalin was 10.58-423 ?g/mL,r=0.999 9,the average recovery was 100.6%.CONCLUSION:This method is rapid,sensitive and accurate.It indicates that geniposide and bai-calin in traditional Chinese medicine preparations can be determinated simultaneously,and the method can be used for quality control.
