Effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with Echinococcus multilocularis
- VernacularTitle:多房棘球绦虫重组BCG-Em Ⅱ/3疫苗对小鼠脾细胞凋亡的影响
- Author:
Mei YANG
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
rBCG-Em Ⅱ/3 vaccine;
Splenocytes;
Apoptosis
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the effects of recombinant BCG-Em Ⅱ/3 vaccine on apoptosis of splenocytes in mice challenged with protoscoleces of Echinococcus multilocularis.Methods:BALB/C mice were immunized with rBCG-Em Ⅱ/3 vaccine by subcutaneous injection or intranasal inoculation.After 8 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection.On the 18th week after infection,mice were killed to separate splenocytes and stimulated with ConA for 16～18h,then apoptosis of splenocytes was detected by Annexin V-FITC staining methods.Results:Apoptotic rate of splenocytes in all groups with or without ConA stimulation was obviously lower than that of PBS group(P
