Induction of neural stem cells into oligodendrocytes
- VernacularTitle:神经干细胞定向分化为少突胶质细胞的实验研究
- Author:
Huimin YANG
- Publication Type:Journal Article
- Keywords:
Neural stem cell;
Thyroid hormone;
Insulin;
Cellular differentiation
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To induce neural stem cells(NSCs)differentiating into oligodendrocytes in vitro and provide experiment foundation for NSCs-derived oligodendrocytes.Methods:The primary NSCs were isolated from endbrain and midbrain of neonatal rats,and then the cells were cultured in vitro.NSCs were induced by thyroid hormone(T3)and(or)insulin,and the effect of T3 and insulin was analysed by comparing the proliferation and the differentiation rate of NSCs and by measuring the prominency of GalC-positive cells.Results:NSCs were induced into oligodendrocytes by thyroid hormone and/or insulin,and different concentration of T3 and/or insulin had different effects on differentiation.Conclusion:Thyroid hormone and insulin can induce NSCs to differentiate mainly into oligodendrocyte and promote the proliferation and maturation of oligodendrocytes.The effect is more outstanding when 40ng/ml T3 is used in combination with 25?g/ml insulin.
