The effects of thoracic epidural block on blood dynamics,neural endocrine and AQP_4 of rabbit with heart failure models
- VernacularTitle:胸段硬膜外阻滞对心力衰竭兔血液动力学、神经内分泌和肺水通道蛋白AQP_4影响
- Author:
Hougang HUANG
- Publication Type:Journal Article
- Keywords:
Thoracic epidural block;
Heart failure;
Blood dynamics;
Neural endocrine;
Aquaporins;
AQP_4mRNA
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the influence of thoracic epidural block on blood dynamics、neural endocrine hormone and the expression of AQP4 mRNA in the lung of rabbit with heart failure models.Methods:60 rabbits which were punctured and cannulated at the thoracic epidural cavity of T5～6 were randomly divided into three groups(n=20):sham operation group(group s),in which the anterior descending branches of left coronary artery were threaded by suture but not tied;heart failure group(groupHF),in which the anterior descending branches of left coronary artery were ligated,0.9%normal sodium 0.5ml/kg was injected to epidural space twice a bay for 4 weeks separately in group S and group HF.TEB group(group TEB),in which 0.1%ropivacaine 0.5ml/kg was injected to epidural space twice a day for 4 weeks after the anterior descending branches of left coronary artery were ligated.After 4 weeks,blood dynamics were monitored directly by insertion through common carotid artery and femoral artery;the level of ANP and ET-1 were mensurated by radioimmunoassay(RIA)and the expression of AQP4 in the lung was quantified by reverse transcription-polymerase chain reaction(RT-PCR).Results:(1)At 4 weeks after ligated LAD,LVEDP was increased significantly and MAP、LVSP、?dp/dt max were decreased significantly in group HF compared with group S.Therapy of TEB was significantly decreased LVEDP and were increased significantly MAP、LVSP、?dp/dt max.(2)Therapy of TEB decreased the level of ANP、ET-1 and increased the expression of AQP4mRNA of the rabbit with heart failure models.Conclusion:Thoracic epidural block have exert anti-heart failure effects by improving blood dynamics parameters,decreased secretion of ANP、ET-1and increased the expression of AQP4mRNA in the lung of rabbits.
