Growth inhibition of nasopharyngeal carcinoma HNE-1 cell strain and effect on its tumorigenic ability by antisense oligodeoxynucleotide targeting telomerase RNA
- VernacularTitle:HTR反义寡核苷酸抑制鼻咽癌细胞生长及对其致瘤能力的影响
- Author:
Qian CAO
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma;
Antisense oligodeoxynucleotide;
Human telomerase RNA;
Nude mice
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the growth inhibition of nasopharyngeal carcinoma cell strain and effect on its tumorigenic ability by AS-ODN targeting telomerase RNA.Methods:Telomerase activity was analysed by PCR-ELISA technique.Proliferation of HNE-1 cells was examined by MTT test and clone formation test.Ultrastructure changes were examined by electron microscope.AS-ODN treated HNE-1 cells were implanted subcutaneously into nude mice to observe tumor growths.Results:When HNE-1 cells were incubated with the AS-ODN targeting telomerase RNA,telomerase activities of HNE-1 cells were significantly inhibited;proliferation of HNE-1 cells was significantly inhibited,which showed a dose-dependent and time-dependent correlation and was sequential specific;swelling or necrosis was found in a small proportion of HNE-1 cells by electron microscopy but no apoptosis and large necrosis area could be found.AS-ODN reduced the tumorigenic ability of HNE-1 cell strain.The tumor formation time was prolonged and tumor growth was slowed down.Conclusion:Through inhibiting the telomerase activity of HNE-1 cell strain,the AS-ODN targeting telomerase RNA can inhibit its growth and reduce its tumorigenic ability.
