Influence of subculture cycle on tube plantlet growth of Dendrobium huoshanense and medium composition
- VernacularTitle:继代周期对霍山石斛试管苗生长及培养基成分的影响
- Author:
Shuhua JIA
;
Di WANG
;
Yuan GAO
;
Yongping CAI
;
Yi LIN
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense C.Z.Tang et S.J.Cheng;
subculture cycle;
plantlet;
medium
- From:
Chinese Traditional and Herbal Drugs
1994;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the best subculture cycle through studying the influence of subculture time on tube plantlet growth of Dendrobium huoshanense and the change of medium composition.Methods Height,tiller,fresh weight,and chlorophyll content of the tube plantlet and pH value,water content,and sugar content of the medium were measured after different cycles of the subculture,the cost of culture medium for subculture was calculated as well.Results The height of the tube plantlet increase 282.86%,the tiller increase by 3.5 times,fresh weight reaches its maximum,chlorophyll content of the tube plantlet almost reaches its maximum after 40 d subculture;while water content and sugar content of the medium are decreased to the lowest point,pH value of medium(
