THE EFFECT OF QUANTUM DOTS ON CYTOCHEMISTRY OF MICE ABDOMINAL CAVITY MACROPHAGES IN VITRO
- VernacularTitle:量子点体外对小鼠腹腔巨噬细胞细胞化学的影响
- Author:
Chengjun ZHAO
- Publication Type:Journal Article
- Keywords:
Quantum dots;
Macrophage;
Culture in vitro;
Cytochemistry;
Mouse
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study the effect of quantum dots(QDs) on macrophage cytochemistry and enzyme activity of mice in vitro. Methods To observe the macrophage biocompatibility with QDs and effect of QDs on PAS reaction,Feulgen reaction,ATPase,acid phosphatase(AcP),alkaline phosphatase(ALP),ANAE,SDH and LDH using inverted phase contrast microscope,fluorescence microscope,and cytochemistry methods were used.Results QDs hadn't effect to macrophage structure in(3.125mg/L) density of QDs,but its cytochemistry and cell enzyme activity had changed differently,that was PAS reaction,Feulgen reaction,AcP,ALP,ANAE and Mg~(2+)-ATP showed positive,and SDH and LDH showed negative.Feulgen reaction,ANAE,ALP and Mg~(2+)-ATP had statistical significance with the control group(P0.05).Conclusion In cell level,macrophage enzymes have changed differently by quantum dots,but QDs don't affect macrophage structure and phagocytosis. QDs can be applied to label living cell in invariably limited dose of QDs in biomedicine area,and cells doesn't have visible influence.
