ISOLATION,CULTURE AND PURIFICATION OF CEREBRAL CORTICAL NEURONS AND GLIAL CELLS FROM THE EMBRYONIC GEKKO
- VernacularTitle:壁虎胚胎大脑皮层神经元和胶质细胞的分离、培养及纯化
- Author:
Yun GU
;
Mei LIU
;
Yan LIU
;
Xiaosong GU
- Publication Type:Journal Article
- Keywords:
Cortical neuron;
Glial cell;
Cell culture;
Gekko japonicus
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of neurons and glial cell culture from embryonic Gekko japonicus cerebral cortex. Methods Embryonic(E15) pallium was dissociated and digesting by trypsin.After counting,cells were seeded in culture flask.The glial cells were obtained by using differential adhesion potential combined with successive passage purified methods,and neurons were obtained by using neurobasal medium supplemented with B27.The cells were fixed and analyzed with immunohistochemic assay. Results After tetra-generation,GFAP positive cells were more than 95% in glial cells cultured condition;Neurons grew well in neurobasal medium,and NF and MAP-2 positive signals co-localized on neurons.After cultured for 10 days,the percentage of neurons was more than 95%.Conclusion The methods of isolation,culture and purification for embryonic Gekko japonicus cortical neurons and glial cells were established and it might be a valuable cell model to further investigate the central nerve system in Gekko japonicus.
