Effects of Arsenic Trioxide on the Expressions of TNF-?,Fas and bcl-2 in Human Lung Adenocarcinoma Cells
- VernacularTitle:亚砷酸对人肺腺癌细胞株TNF-?、Fas、bcl-2表达的影响
- Author:
Xiaowei YE
;
Huili YE
;
Yao CHEN
;
Linxiang CHEN
;
Xuexin CHEN
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide(As_2O_3);
Lung Adenocarcinoma Cell Line(LAC);
TNF-?;
Fas;
bcl-2
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of arsenic trioxide(As2O3)on the expressions of TNF-?,Fas and bcl-2 in lung adenocarcinoma cells(LAC)and to explore the mechanism of arsenic trioxide inducing apoptosis.Methods The expressions of TNF-?,Fas and bcl-2 in lung adenocarcinoma cells pretreated by arsenious acid were determined by the double antibody sandwich ABC-ELISA method.Results Compared with the control group,As2O3 showed no effects on the contents of bcl-2 in lung adenocarcinoma cells after 72 hours treatment,but increased the contents of TNF-? and Fas significantly,and the effects in different concentration groups had significant differences.The protein expressions of TNF-? and Fas showed a tendency of concentration-dependent increasing.Conclusions The results suggest that As2O3 induces the apoptosis of LAC cells possibly by up-regulating the expression of TNF-? and Fas.
