Purification and assay of flavonoids in glycyrrhiza
- VernacularTitle:甘草中黄酮的纯化方法和含量测定
- Author:
Junsong LI
;
Desheng XU
;
Yi FENG
;
Lihua CHEN
- Publication Type:Journal Article
- Keywords:
Glycyrrhiza;
flavone;
polyamide
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the process parameters of adsorbing and separating the total flavones of Radix et Rhizoma glycyrrhiae and its assay. METHODS: Chalcone that flavanone in Licorice root was converted into in alkaline condition was adopted as index and niaringoside as control,through polyamide treatment,alkaline colorimetty was used to determine chalcone content. RESULTS: Under the condtion,namely, ratio of quantity of Licorice root to polyamide was 2:1,ratio of column diameter to column heighth was 1:7,70%ethanol used to eluate,flavone extract was up to 45%with 90%eluting yield.The recoveny of assaying was 98.17%(RSD=2.00%). CONCLUSION: The purification and assay of Licorice flavonoids are reliable and efficacious.
