Dynamic observation on subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis
- VernacularTitle:多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后脾细胞亚群的动态观察
- Author:
Wengui LI
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
Recombinant BCG-EmⅡ/3 vaccine;
Recombinant BCG-Em14-3-3 vaccine;
Subsets of splenocytes
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To dynamically observe changes of subsets of splenocytes in mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em).Methods:BALB/C mice were intranasally vacci- nated with the vaccine and killed to get spleen at 0,2,4,6,8,10,12,14,16 and 18w of immunization respectively.Splenocytes were.separated to measure subsets of CD_4~+ and CD_8~+T cells by FACsort,PBS served as control.Results:In the groups of im- munization,CD_4~+ and CD_8~+subsets increased obviously at 2～12w and 2-18w respectively,which reached the highest level at 6 and 10w respectively.Conclusion:The number of CD_4~+ subsets of splenocytes increases remarkably in the early stage (2～6week)by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine.
