Effects of Astragalus Polysaccharide on Differentiation and Maturation of Dendritic Cells in Vivo
- VernacularTitle:黄芪多糖对小鼠树突状细胞前体的体内诱导研究
- Author:
Weiwei HU
;
Guoqing ZANG
;
Yongsheng YU
- Publication Type:Journal Article
- Keywords:
dendritic cells;
astragalus polysaccharide;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of astragalus polysaccharide,a component of an aqueous extract of Astragalus Membranaceus roots,on differentiation and maturation of dendritic cells in vitro. Methods 30 BALB/c mice were randomly divided into three groups,normal control group,100,200 mg/kg APS intraperitoneal injection groups. After one week,weight the mouse spleen,account the splenetic index. Collect the mouse bone marrow cell,induced and cultured with rmGM-CSF and rmIL-4. With inverted microscope to investigate the morphous of DC cell. The phenotypes of DC were detected by flow cytometry and the expression of the GM-CSF protein in serum was tested by ELISA. Results Astragalus polysaccharide injection had obvious effects on the spleen weight of mice. The degree of CD11c and MHC-Ⅱ expression in 100 mg/kg and 200 mg/kg groups on flow cytometry were advanced significantly compared with that in normal control group,but the degree of CD80 and CD86 was not increased. And the expression of the GM-CSF protein in serum in 100 mg/kg group and 200 mg/kg group were both not increased significantly compared with nomal control group. Conclusion The intraperitoneal injection of astragalus polysaccharide could stimulate the proliferation of the pre DC in bone marrow. The angtigen presentation of DC might be enhanced,but this effects was not positive correlation with concentration of GM-CSF.
