Determination of Sinapine Thiocyanate in the Baixi Kechuan Cream by HPLC
- VernacularTitle:白细咳喘膏中芥子碱硫氰酸盐的含量测定
- Author:
Ling YAN
;
Xiaoling WANG
;
Luoluo CONG
- Publication Type:Journal Article
- Keywords:
HPLC;
Semen Sinapis albae;
Sinapine thiocyanate;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of Sinapine thiocyanate in the Baixi Kechuan Cream by HPLC and to lay foundation for establishing the quality control standard of the preparation. Methods The HPLC system consisted of the column of DIKMA inertsil (pH=3,5 ?m,250 mm?4.6 mm). The acetonitrile and 3% acetic acid were used as the mobile phase A and B,respectively,and gradient elution was used. The detective wavelength was 326 nm and the flow rate was 1.0 mL/min. The column temperature was room temperature. Results The method was linear within the range of 0.8～4.0 ?g (r=0.999 6,n=5). The average recovery was 100.83%,and RSD=1.95% (n=5). Conclusion The method is quick,simple,highly reproductive and steady,and it can be used for quality control of the preparation.
