Content Determination of Rutin in Xueguankang Tea by Reversed Phase HPLC
- VernacularTitle:RP-HPLC法测定血管康袋泡茶中芦丁的含量
- Author:
Yanmei LUO
;
Zhenghua JIAO
;
Zhimao CHAO
- Publication Type:Journal Article
- Keywords:
Xueguankang Tea;
rutin;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of rutin in Xueguankang Tea. Methods RP-HPLC method and C18 column were used for determination. Methanol-water-acetic acid (40∶60∶1) was the mobile phase with a flow rate of 1.0 mL/min. The detection wavelength was set at 257 nm. Results There was a good linear relationship of rutin within the range of 0.06～0.3 ?g with r=0.999 2. The average recovery of rutin was 98.48 % with RSD=0.87% (n=5). Conclusion This method is convenient,sensitive,accurate and reproducible,and can be used for the quality control of Xueguankang Tea.
