Selecting of good strain breeding in Glycyrrhiza uralensis(Ⅰ)——AFLP Analysis on genetic basis for four Glycyrrhiza uralensis
- VernacularTitle:乌拉尔甘草优良品系选育研究(Ⅰ)——4个来源甘草遗传基础的AFLP分析
- Author:
Chengming ZHOU
;
Bin XU
;
Jintun ZHANG
;
Wenyuan GAO
- Publication Type:Journal Article
- Keywords:
Glycyrrhiza uralensis Fisch.;
selecting for good strain breeding;
DNA fingerprinting
- From:
Chinese Traditional and Herbal Drugs
1994;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Study the genetic basis of four plants in Glycyrrhiza L.and apply the amplified fragment length polymorphism(AFLP)molecular marker technique to the selecting of good strain breeding of Glycyrrhiza uralensis.Methods The DNA polymorphism,fingerprinting,and UPGMA ana-lysis of four cultivated species in G.uralensis from Minqin,Kashi,Akesu,and Inner Mongolia were detected by AFLP technique.Results Eight primer combinations were screened from 64 primer combinations to analyze DNA polymorphism and the DNA fingerprintings were generated by primer combination E-AAC/M-CAG.UPGMA Analysis showd that all the studied populations were clustered into four groups and had different relationships.Conclusion The results show that "Minqin No.1","Kashi No.1",and "Akesu No.1" have inimitable gene structure and should be studied more as new breeding resource.