Determination of entrapment efficiency of bulleyaconitine A multivesicular liposomes
- VernacularTitle:草乌甲素多囊脂质体包封率的测定
- Author:
Weigen LU
- Publication Type:Journal Article
- Keywords:
bulleyaconitine A;
multivesicular liposomes;
entrapment efficiency;
centrifugalization
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method of determining the entrapment efficiency of bulleyaconitine A multivesicular liposomes.METHODS:Bulleyaconitine multivesicular liposomes and its free bulleyaconitine were separated by centrifugalization.To calculate the entrapment efficiency,total and free bulleyaconitine were assayed by HPLC.RESULTS:At low speed,the rate of rotation did not affect the determination of entrapment efficiency.The average entrapment efficiency of three lot samples was 87.12%.CONCLUSION:Entrapment efficiency of bulleyaconitine multivesicular liposomes could be evaluated simply and quickly by centrifugalization.
