Determination of terpenelactones in Yinxing Tongzhi Dripping Pills by HPLC-ELSD

VernacularTitle:HPLC-ELSD测定银杏酮酯滴丸中萜类内酯的含量
Author: Qin LI ; Fengxian MA ; Tiefeng CHENG
Publication Type:Journal Article
Keywords: Yinxing Tongzhi Dripping Pills; terpenelactones; HPLC; ELSD
From: Chinese Traditional Patent Medicine 1992;0(06):-
CountryChina
Language:Chinese
Abstract: AIM:To determine terpenelactonesines content in Yinxing Tongzhi Dripping Pills(ginkgoflavone,ginkgolide).METHODS:HPIC with evaporative light scattering detector(ELSD) was established to determine terpenelactones content in Yinxing Tongzhi Dripping Pills..The analysis was carried out on Angilent Hypersil ODS(5 ?m,4 mm?250 mm) column.The mobile phase consisted of THF-MeOH-H_2O(10:20:70).The operating conditions of the ELSD were a nebulizer nitrogen,flow-rate of 2.51 L/min and an evaporator tube temperature of 103(?C).RESULTS:No interference was found in the terpenelactones peak position.The method was simple,with a good linear relationship.The relative standard deviation of reproducibility about BB、GA、GB、GC was 1.33%,(1.79%,)1.92%,1.58% respectively and the average recoveries were 97.0%,96.5%,96.3%,97.6%,respectively.CONCLUSION:The method can be used for quality control.