Determination of contents of salvianolic acid B and danshensu and protocatechuic aldehyde of Shuangdan Oral Solution by RP-HPLC
- VernacularTitle:RP-HPLC测定双丹口服液中丹酚酸B、丹参素和原儿茶醛的含量
- Author:
Hongya LIU
;
Yong LEI
;
Dajian YANG
- Publication Type:Journal Article
- Keywords:
Shuangdan Oral Solution;
salvianolic acid B;
danshensu;
protocatechuic aldehyde;
HPLC;
quantitative determination
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for determining the contents of salvianolic acid B,danshensu and protocatechuic aldehyde,the effective components of Shuangdan Oral Solution(Radix et Rhizoma Salviae miltiorrhizae,Cortex moutan) by HPLC.METHODS:Experiment was carried out on a Phenomenex Luna C_(18) column(4.6 mm?150 mm,5 ?m) with a mobile phase methanol-acetonitrile-methane acid-water(30:10:1:59) at a flow velocity of 1.0 mL/min and detection wavelength at 286 nm for salvianolic acid B,a mobile phase methanol-acetonitrile-methane acid water solution [HCOOH:H_2O(2:98)](9:4:87) at a flow velocity of 1.0 mL/min and detection wavelength at 281 nm for danshensu and protocatechuic aldehyde.RESULTS:The average recoveries of salviano-(lic) acid B,danshensu and protocatechuic aldehyde were 98.6%(RSD=1.91%,n=6),99.2%(RSD=2.2%,n=6) and 97.3%(RSD=2.5%,n=6) respectively.CONCLUSION:The results show that this method is easy to operate and accurate,and can be used to determine the contents of the three effective components in Shuangdan Oral Solution.
