Quantitative determination of paeoniflorin and puerarin in Rg soft-exact by HPLC
- VernacularTitle:HPLC同时测定广东汤中葛根素和芍药苷的含量
- Author:
Yan LIU
;
Shunhang LIU
;
Jiali JIA
;
Bo XU
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
pseudoephedrine;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This paper described a HPLC method.Paeoniflorin and puerarin in Rg soft-exact(Radix Puerariae lobatae,Redix Paeoniae alba,etc.) were simultaneously determined.METHODS:A simplified HPLC procedure was developed.A 4.6 mm?250 mm Alltima C_(18) column with a mobile phase consisting of NaH_2PO_4(20 Mm,pH 2.4)-Acetonitrile:Tetrahydrofuran(900:120:5) was used.The flow rate was 1.00 mL/min.It was detected at 320 nm.RESULTS:Paeoniflorin and puerarin were well separated.The average recoveries of paeoniflorin and puerarin were 98.10% and 101.2% respectively.CONCLUSION:The result shows that this method is convenient,rapid and accurate.
