Expression and purification of streptococcus pneumoniae virulence factor PspA
- VernacularTitle:肺炎链球菌毒力因子PspA的原核表达、纯化及鉴定
- Author:
Wenchun XU
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumonia;
PspA;
Vaccine
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain purified PspA produced by prokaryotic expression system.Methods:Template DNA was isolated from cultured Streptococcus pneumonia TIGR4.By gene recombination technology in vitro,sequence encoding PspA antigen epitope was cloned into PET-32(a) expression vector.After being confirmed by sequencing,expressed antigen protein was identified by SDS-PAGE and Western blot.Results:The DNA sequence analysis confirmed that the cloned PspA gene was according to GenBank data.The PspA fusion protein was proved by Western blot.Conclusion:A highly expressed recombinant PspA protein was successfully obtained.
