Changes on subsets of T cells in the spleen of mice by immunization with mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine against Echinococcus multilocularis
- VernacularTitle:多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗诱导小鼠脾脏T细胞亚群的变化
- Author:
Jun TANG
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
Recombinant BCG-EmⅡ/3 vaccine;
Recombinant BCG-Em14-3-3 vaccine;
Subsets of spleen cells
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate changes in subsets of T cells in the spleen of mice by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em)and against challenge with Em protoscoleces.Methods:BALB/C mice were subcutaneously and intranasally vaccinated with mix recombinant BCG vaccines respectively,then challenged with Em protoscolexes on 8w of vaccination and killed on 18w of infection to get spleen.Spleen cells were separated to measure subsets of CD4+ and CD8+T cells by FACsort,Blank vector,BCG and PBS served as control.Results:In the groups of immunization,CD4+subset increased significantly,as well as the ratio of CD4+/CD8+subset,but CD8+ subset had no change,CD4+ subset and ratio of CD4+/CD8+subset in the subcutaneous group were higher than these in the intranasal group.Conclusion:CD4+T cells subset may bear a part in protection induced in mice by mixed recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine against challenge with Em protoscoleces.
