Experimental Study on Eqi Compound Prescription Induced Apoptosis of Acute Myelocytic Leukemia HL-60
- VernacularTitle:莪芪抗瘤方剂诱导白血病HL-60细胞凋亡的血清药理学研究
- Author:
Jun HAO
;
Che CHEN
;
Long CHEN
- Publication Type:Journal Article
- Keywords:
Eqi compound prescription;
acute myelocytic leukemia;
apoptosis;
Caspase-3;
Bcl-2
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the apoptotic effect of Eqi compound prescription (contained-herb serum) on cute myelocytic leukemia HL-60 cell, which is relative to intracellular Ca2+, Caspase-3 and Bcl-2. Methods According to serum pharmacology, HL-60 cells were exposed to 10% concentrations of contained-herb serum for 24, 48, 72 and 96 hours respectively. Cells were observed under a fluore-scence microscope. SubG1 DNA was examined by flow cytometer. Intracellular Ca2+ concentration was measured by fura-2 fluorescence load method. Caspase-3 enzymatic activity were measured by colorimetry. Bcl-2 gene expression were measured by LSAB. Results The contained-herb serum could induce apoptosis of HL-60 cells. Intracellular Ca2+ concentration of treatment with Eqi was higher evidently than that of control (P
