Determination of the Contents of Berberine Hydrochloride in Spring Rain Burns Gels
- VernacularTitle:春雨烧伤凝胶剂中盐酸小檗碱的含量测定
- Author:
Weiqiong REN
;
Jianping LIAO
;
Chengyuan WANG
- Publication Type:Journal Article
- Keywords:
spring rain burns gels;
berberine hydrochloride;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination the contents of berberine hydrochloride in spring rain burns gels. Methods Analytical column was Kromasil C18 (4.6 mm?250 mm, 5 ?m) column with a mobile phase of Acetonitrile-1%H3PO4-Triethylamine (24∶76∶0.76), the detective wavelength was 345 nm, the flow rate was 1.0 mL/min and column temperature was 30 ℃. Results A good linearity was obtained in the range of 0.088～0.440 ?g (r =0.999 7), and the average recovery was 98.13% with RSD=0.77% (n=6). Conclusion The method is rapid and reliable, and can be used for the quality control of spring rain burns gels.
