In vitro study of Ganli Injection on reversing acquired multidrug resistance of human hepatocellular carcinoma
- VernacularTitle:肝力注射液逆转人肝癌细胞获得性多药耐药的体外研究
- Author:
Wei GU
;
Xiaofeng ZHAI
;
Yani ZHANG
;
Changquan LING
- Publication Type:Journal Article
- Keywords:
Ganli Injection;
matrine;
ligustrazine hyydrochloride;
hepatocellular carcinoma (HCC);
multidrug resistance (MDR)
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the reversal effect on acquired multidrug resistance (MDR) of human hepatocellular caricinoma (HCC) by Ganli Injection. Methods Proliferative inhibition rate of HCC BEL-7402/5-Fu cell line and reversal index of 5-Fu acquired MDR were measured by MTT assay. Cell morphological changes were observed under inverted microscope and Wright-Giemsa stain method. Fluorescence intensity of Adriamycin (ADM) was detected by Flow cytometry. Results HCC BEL-7402/5-Fu cell line exhibited cross-resistance to general chemotherapeutics in clinic. Proliferative inhibition rates of HCC BEL-7402/5-Fu cell line treated for 72 h by Ganli Injection at the (matrine) concentration of 0.230, 0.115, 0.058 mg/mL were (9.25?2.38)%, (8.46?1.90)%, and (4.23?2.05)%, respectively. The corresponding reversal indexes to 5-Fu were 5.50, 3.83, and 2.25. Pre-treatment of Galli Injection at the concentration of 0.230 mg/mL for 2 h could increase the accumulation of ADM in cells to 38.61%.Conclusion Ganli Injection has the effect on reversing 5-Fu acquired MDR of human hepatocellular carcinoma in vitro.
