THE EFFECT OF CGRP ON THE EXPRESSION OF CREB mRNA IN RAT HIPPOCAMPUS AND PARIETAL CORTEX DURING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION
- VernacularTitle:降钙素基因相关肽对局灶性脑缺血再灌注大鼠脑cAMP反应元件结合蛋白mRNA表达的影响
- Author:
Zhenghong ZHANG
;
Peng QU
;
Yuli LIU
;
Baohui ZHANG
;
Xiubin FANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Calcitonin gene related peptide;
c-AMP response element binding protein;
In situ hybridization;
Rat
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of calcitonin gene related peptide(CGRP) on the expression of cyclic AMP response element binding protein(CREB) mRNA in rat hippocampus and parietal cortex during focal cerebral ischemia and reperfusion(I/R). Methods Focal cerebral ischemia/reperfusion model was induced by occluding of the right middle cerebral artery using the intraluminal suture method.Hybridization in situ experiment was used to detect the expression of CREB mRNA in the ipsilateral hippocampal CA1 region and parietal cortex during different reperfusion periods.The positive product of CREB mRNA was analyzed by image analysis system. Results There was a distinct expression of CREB mRNA in right hippocampal CA1 region and parietal cortex in sham group.The absorbency of CREB mRNA positive product reduced in I/R group as compared to sham group,while it increased in CGRP group than I/R group(P
