THE REGWATION OF GANGLIOSIDE ON PKC PATHWAYS AND ITS PROTECTIVE EFFECT ON SERUM-DEPRIVED INJURY IN PC12 CELLS
- VernacularTitle:神经节苷脂调节PKC信号通路及其对PC12细胞去血清损伤的保护作用
- Author:
Ying SONG
;
Meng LI
;
Jianren LIU
;
Sa YU
;
Jicheng LI
- Publication Type:Journal Article
- Keywords:
Cell differentiation;
Serum-deprived;
Monosialoganglionside(GM1);
Protein kinase C(PKC) pathway;
Western blotting;
Rat pheochromocytoma cell(PC12 cell)
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determinted whether GM1 had a protective effect on injury induced by serum-deprivation and the possible mechanism in PC12 cells. Methods The viability of PC12 cells was quantified by MTT after serum-deprivation.The number of apoptotic cells and necrotic cells were determined by Hoechst 33258/PI staining.And the change of PKC protein expression on PC12 cells' membrane and cytosols was detected by Western blotting. Results 1.The viability of PC12 cells decreased after serum-deprivation and the serum-deprivation for 24 hours was chosen as an injury model in this research.Most of the PC12 cells presented apoptosis 24 hours after serum-deprivation.In addition,the PC12 cells' cytosols PKC protein decreased,while the PC12 cells' membrane PKC protein increased significantly,and this result suggested PKC's translocation to membrane and its activation.2.The viability of PC12 cells preincubated with GM1 in high concentrations(10,1,0.1?mol/L) increased significantly and GM1 protected PC12 cells from apoptosis after serum-deprived injury.GM1 reduced the damage of serum-deprivation on PC12 cells and inhibited PKC protein translocation after injury.3.The repair function of GM1 was effective to neuronal resume after serum-deprived injury.Conclusion Neuroprotective effects of GM1 on serum-deprived injury may be partly mediated through the regulation of PKC pathways and it is helpful for the recovery after injury.
