THE EFFECT OF MACROPHAGE-CONDITIONED MEDIUM ON THE GROWTH OF PRIMARY MICE MYOBLASTS
- VernacularTitle:巨噬细胞条件培养液对原代培养小鼠成肌细胞生长的影响
- Author:
Danyi SHU
;
Junmin TANG
;
Yan TANG
;
Feng LI
- Publication Type:Journal Article
- Keywords:
Macrophage;
Myoblast;
in vitro;
Immunocytochemistry;
Mouse
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of macrophage-conditioned medium on the growth of mouse myoblasts. Methods After a 3-day intraperitoneal injection with a mixture of muscle homogenate and starch,macrophages were obtained from mouse peritoneal cavity and cultured for 48 hours in serum-free DMEM/F12.Then the cultured medium was harvested.Myoblasts were obtained from newborn mice skeletal muscle and grown in DMEM/F12 supplied with 10% FBS for 36-48 hours,and then exposed to 0.5% FBS medium with or without macrophage-conditioned medium.Cells were harvested at 72 hours later,their growth was observed with Wright staining,LDH cytochemistry and immunocytochemistry. Results Macrophage-conditioned medium can maintain the normal morphology and the activity of myoblasts grown in low concentration of serum and promote their proliferation.There was a significant difference between average absorbance and integral absorbance of LDH positive cells of the two groups(P
