Effect of Naringin on Proliferation and Differentiation of 3T3-L1 Preadipocyte
- VernacularTitle:柚皮苷对前脂肪细胞3T3-L1增殖和诱导分化的影响
- Author:
Xinying LIU
;
Lian ZHOU
;
Ruiyan LIANG
;
Liuying CAO
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
Naringin;
3T3-L1 cells;
Proliferation and differentiation;
Gene expression
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of naringin on proliferation and differentiation of 3T3-L1 preadipocyte,and to investigate its possible mechanism.Methods The cultured 3T3-L1 cells were pretreated with naringin,and then MTT method was used to detect the proliferation of the cells,oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.The expression of PPAR? 2 and C/EBP? mRNA associated with adipocytes differentiation were detected by reverse transcription PCR(RT-PCR).Results Naringin suppressed the proliferation and differentiation of 3T3-L1 preadipocytes and down-regulated the expression of PPAR? 2 and C/EBP? mRNA.Conclusion Naringin can inhibit the proliferation of 3T3-L1,and can suppress their differentiation through down-regulating the expression of PPAR? 2 and C/EBP? mRNA.
